How does the GeneSTAT System work?
The GeneSTAT monitors the production of DNA copies during each PCR amplification cycle via fluorescent dyes. Fluorescence measured during a cycle is indicative of the amount of copied target DNA present at that time.
What is a GeneSTAT Test Module?
The GeneSTAT Test Module is a plastic, single-use, disposable closed-system unit in which all processes needed for a successful PCR analysis happen automatically, from sample preparation -- including (when necessary) reverse transcriptase for making copy DNA from RNA -- to DNA amplification. All necessary reagents are contained therein. After adding a sample to it, the Test Module is simply inserted into the GeneSTAT Analyzer and a button pushed to initiate a complete reaction.
Can a multiplex assay be done with GeneSTAT?
A GeneSTAT Test Module can process up to four targets at once. So, multiple tests may be done at the same time with a sample and an internal control included for confidence in the results.
How long will it take to run an assay?
From sample processing to a final results takes 60 minutes or less with GeneSTAT.
What assays can be run on the GeneSTAT System?
Any PCR test may be rendered usable in the GeneSTAT System. Assays made by DxNA can be immediately run on the GeneSTAT system. These are listed in the products section of the DxNA website.
What about custom tests for the GeneSTAT?
Our technology and experience is enthusiastically available for making custom GeneSTAT assays suited to your needs. A complete customization will include creating specific PCR primers and probes, and then establishing optimal GeneSTAT PCR conditions for the most robust, specific, and sensitive assay. You will have direct access to DxNA scientific and quality assurance staff at all times during the development process.
What sorts of samples may be processed in the GeneSTAT Test Module?
A wide variety of sample types can be looked at with GeneSTAT. These include mucous as throat or tracheal swabs, whole blood, serum, plasma, purulent discharges, other animal fluids, stool samples and cloacal swabs.
A feature being integrated into GeneSTAT is a novel, patent-protected high-intensity sonicator in which samples are uniquely not touched by a tine (a fork-like implement) to break apart specimens. This means that closed-system integrity can be maintained. With it, the spectrum of samples that can be analyzed by GeneSTAT expands to include more solid biological materials like organ biopsies, cervical and other sorts of cell samples and varied plant materials. Work shows that the sonicator can well homogenize and solubilize insects, highly desirable for looking at vector-borne diseases like West Nile Virus.
Even though the GeneStat Test Module is closed, can copy DNA still be removed for running on a gel, for cloning or for other activities?
The final amplified DNA product can be removed from the Test Module by opening it with a scalpel and then removing the contents from the reaction wells with a gel loading pipette tip.
What fluorescent dyes can be used on the GeneSTAT System?
Common fluorescent detection dyes that attach to DNA as it is made like FAM (fluorescein), Texas Red, TAMRA and SYBR Green can be readily employed in GeneSTAT. Modified DNA probes that yield a fluorescent signal when these attach to complementary DNA made during PCR can also be used.