Genetic, Molecular Analyses

Features of GeneSTAT RT-PCR include

• The GeneSTAT Analyzer performs PCR and can be used anywhere, indoors or outdoors. Its design incorporates circuitry to accommodate external battery operation. Tests indicate that operational reliability includes an exceptional 10,000-hours mean-time-between-failure rate.
• Simple, one-button operation: After inserting the test module into the analyzer, all subsequent processes from initial sample processing to the display of a final result automatically happen by simply pressing a button.
• Elimination of the need for specimen preservation or transportation: Since samples may be processed immediately, sample degradation associated with lengthy transport times becomes a virtual non-issue with GeneSTAT. This is especially of benefit with RNA, which is notorious for its rapid breakdown.
• Remote Data Transmission, Data Storage and Screen Display: Assay data may be stored inside the GeneSTAT Analyzer. The Analyzer also has a USB port and circuitry that makes it possible to see detailed results on a computer or to transmit data wirelessly to locations far away. This means that results may be sent from isolated or inadequately staffed locales to places where qualified professional counsel is available. Results are also visually available on an LCD screen.

What is PCR?

PCR can detect DNA unique to an individual or species. It works by copying small, specific pieces of DNA from its target. Beginning with a single molecule, PCR can generate billions of like DNA pieces, enough to become easily identified and otherwise be analyzed.

An important feature of GeneSTAT is that amplified DNA is detected as it accumulates in the reaction – i.e., as copies are made during each amplification cycle.

While PCR can only done with DNA, RNA is the sole genetic material in many disease-causing viruses. These RNA viruses include more than 300 known human pathogens, most emerging disease agents and many of concern regarding bioterrorism. Fortunately, PCR can be combined with “reverse transcriptase” chemistry, such that target pieces of RNA are copied into DNA that is then amplified by PCR. This enables the identification disease-causing RNA viruses like Influenza, West Nile, Hepatitis C, Ebola, Yellow Fever and the Human Immunodeficiency.